DNA 표지를 이용한 딸기 국내 육성 품종 판별

• 영문명: Identification of Korean Strawberry Cultivars using DNA markers
• 발행기관: 한국육종학회
• 저자명: 조강희(Kang-Hee Cho) 노일래(Il Rae Rho) 조용섭(Yong Seop Cho) 박부희(Pue-Hee Park)
• 간행물 정보: 『한국육종학회지』Vol.40 No.4, 401~407쪽, 전체 7쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2008.12.30

영문 초록

This study was conducted to develop the DNA markers for identification of the strawberry cultivars in Korea and Japan. We developed fifteen cleaved amplified polymorphic sequence (CAPS) markers based on the Fragaria gene sequences. Among them six CAPS markers showed polymorphism exclusively in one cultivar. Five CAPS markers (ANR-MspI, ANRBamHI, ACO-HinfI, DFR-AseI, FGT-MspI) provided enough polymorphism to identify eight Korean strawberry cultivars except for ‘Maehyang’ and ‘Sunhong’. To complement the fifteen CAPS markers, we selected another fifteen sequence-related amplified polymorphism (SRAP) and one of them, me1/em5_460bp marker, made it possible to discriminate between ‘Maehyang’ and ‘Sunhong’. Therefore, application of the five CAPS markers and one SRAP marker were sufficient to identify the nineteen Korean and Japanese strawberry cultivars. These markers could be used practically for cultivar identification of Korean and Japanese strawberry.

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키워드

strawberry cultivar identification DNA markers CAPS SRAP