벼 Ds 삽입변이 pooling 계통들의 FST 및 유전자형 분석

• 영문명: Analysis of Genotype and Flanking Sequence Tagged from pooled Ds Insertional lines in rice
• 발행기관: 한국육종학회
• 저자명: 안병옥(Byung-Ohg Ahn) 김정호(Jeong-Ho Kim) 지상혜(Sang-hye Ji) 윤도원(Doh-Won Yun) 박용환(Yong-Hwan Park) 지현소(Hyeon-So Ji) 은무영(Moo-Young Eun) 이기환(Gi-hwan Lee) 서석철(Seok-Cheol Suh) 이명철(Myung-Chul Lee)
• 간행물 정보: 『한국육종학회지』Vol.40 No.4, 387~393쪽, 전체 7쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2008.12.30

영문 초록

Over 7 individual rice (Oryza sativa L.) plants per a line were sowed and sampled by pooled sampling method for genomic DNA extraction. The 5,400 flanking sequence tags (FSTs) were analysed by adaptor PCR and direct sequencing. FST analysis showed that the intragenic FSTs, the intergenic FSTs, and the original insertional sequences including hot spot covered 48.1% (2,597), 25.6% (1,383), and 25% (1,350), respectively. The 2,597 intragenic FSTs were used for genotyping to determine whether they are heterozygous or homozygous, and 1,393 core lines were selected. Among them, 422 knockout genes were distributed on chromosome 3, while 56 - 157 intragenic FSTs scattered on other chromosomes. Among 1,393 FSTs, known genes such as transcription factor covered 59.4% (827), while unknown genes such as expressed protein covered 40.6% (566). RT-PCR indicated that some core lines had no expression or decreased expression level in their knockout genes. It means that core lines are very useful knockout lines for functional genomic studies.

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서 언

재료 및 방법

결과 및 고찰

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키워드

Rice /(Oryza sativa L.) Ac/Ds FST Genotype