Mapping of the Reduced Culm Number Trait in Rice (Oryza sativa L.) rcn10(t) Mutant

• 발행기관: 한국육종학회
• 저자명: Un-Sang Yeo Jong-Hee Lee Choon-Song Kim Meong-Gi Jeon Tae-Yong Oh Chang-Deok Han Mun-Sik Shin Byeong-Geun Oh
• 간행물 정보: 『한국육종학회지』Vol.40 No.3, 223~227쪽, 전체 5쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2008.09.30

영문 초록

In rice, tillering is an important trait determining yield. To study tillering at the agricultural and molecular aspects, we have examined a spontaneous rice mutant that showed reduction in the number of culms. The mutant was derived from a F 6 line of the cross of Junambyeo*4 / IR72. It could produce, on average, 4 tillers per hill in the paddy field while wild-type plants usually have 15. Except the reduced culm numbers, they also show pale green phenotypes. The phenotypes of this mutant were co-segregated as the monogenic Mendelian ratio (χ 2 = 0.002, p = 0.969). In order to locate a gene responsible for the rcn phenotype, the mutant with the japonica genetic background was crossed with Milyang21 of the indica background. Bulked segregant analysis was used for rapid determination of chromosomal location. Three SSR markers (RM551, RM8213, and RM16467) on chromosome 4 were genetically associated with the mutant phenotype. Each of the 217 F 2 plants was genotyped with simple sequence length polymorphisms. The data showed that RM16572 on chromosome 4 was the closest marker that showed perfect co-segregation among the F 2 population. We suggest the new rcn gene studied here name as rcn10 t because there was no report which exhibit a rcn phenotype with a pleiotropic effect of pale green (chlorophyll deficiency), and mapped at same position on chromosome 4.

목차

INTRODUCTION

MATERIALS AND METHODS

RESULTS AND DISCUSSION

키워드

Reduced culm number (rcn) Mutant Rice Mapping SSR marker