Inheritance of Herbicide (glufosinate) Resistance in Transgenic Rice Plant through Anther Culture

• 발행기관: 한국육종학회
• 저자명: Hyeon-Jung Kang Hyun-Soon Kim Jeong-Kwon Nam Young-Tae Lee Seung-Yeob Lee Chung-Kon Kim
• 간행물 정보: 『한국육종학회지』Vol.40 No.3, 211~214쪽, 전체 4쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2008.09.30

영문 초록

Haploid system by anther culture allows the development of homozygous lines when doubled. The response of anther culture to Basta (glufosinate) resistance was investigated on transgenic plants (cv. Anjungbyeo) in order to identify inheritance of bar gene associated with Basta. Most of the regenerated transgenic plants were sterile, and only a few plants produced viable seeds (A 1 ) in the greenhouse. The bar gene was analysis by PCR in basta resistant transgenic plant (TA 0 ). The transgenic seeds (A 1 ) were significantly germinated in Basta solution compared with non-transformed seeds. As a result of anther culture, in regenerated haploid plants, segregation ratio was 1:1 in five of eight cross combinations. In diploid plants, segregation ratio was 1:1 in seven of eight cross combinations. Although there was some differences in the cross combinations, most of the combinations had 1:1 segregation ratio which supports the theory. The difference may be a result of the small sample size or the difference of anther culture response caused by genotypic difference. Hence, when many cross combinations were anther-cultured the results would support the theory.

목차

INTRODUCTION

MATERIALS AND METHODS

RESULTS AND DISCUSSION

키워드

rice transformation herbicide (glufosinate) resistance anther culture