당근의 약배양으로부터 직접 및 간접 배형성을 통한 식물체 생산 체계 확립

• 영문명: Establishment of Plant Production System through Direct and Indirect Embryogenesis from Anther Culture of Carrot
• 발행기관: 한국육종학회
• 저자명: 김미영(Miyoung Kim) 형남인(Nam-In Hyung)
• 간행물 정보: 『한국육종학회지』Vol.46 No.4, 372~380쪽, 전체 9쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2014.12.30

영문 초록

We tried to develop the protocol for embryogenesis and plant regeneration from anther culture of carrot (Daucus carota L.) genotype ‘S&P2342’. Anthers were cultured on MS medium with B5 vitamins containing different combinations of 2,4-D and NAA for 18 weeks in the dark. The best induction of callus and embryo was obtained in the medium containing 0.1 mg/L 2,4-D and 0.1 mg/L NAA, on which 22.0% callus and 2.0% embryo were induced. When primary embryos induced directly from anther culture were transferred to the regeneration medium, secondary embryos were initiated from primary embryos after 4 of culture and 62.5% converted into plantlets after 8 weeks of culture. The plantlets with true leaves were obtained after 12 weeks of culture. When the calli derived from anther culture were transferred to the regeneration medium, 38.8% of the calli produced primary embryos and plantlets after 8 weeks of culture. The plantlets with 2 or more leaves cultured on the regeneration under the different light intensity for the growth of in vitro plantlets. The plantlets cultured at 100 μmol･m-2･s-1 showed the highest growth rate. For the acclimatization, the in vitro plantlets with 4 or more leaves cultivated under the different light intensity and temperature, respectively. The survival rate and growth of plantlets was best at 15℃ and 100 μmol･m-2･s-1, respectively. The plants were successfully acclimatized and had a normal phenotype. The anther culture system could be used to prepare the doubled haploid lines as an appropriate breeding material for F1 hybrid breeding program.

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서 언

재료 및 방법

결과 및 고찰

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REFERENCES

키워드

Carrot Anther culture Embryo Callus Plant regeneration