가뭄저항성벼(Agb0103)의 분자생물학적 특성과 안전성 평가

• 영문명: Molecular Biological Characteristics and Biosafety Assessment for Drought-tolerant Transgenic Rice (Agb0103)
• 발행기관: 한국육종학회
• 저자명: 오성덕(Sung-Dug Oh) 이기종(Kijong Lee) 원소윤(So Youn Won) 손수인(Soo-In Sohn) 이시명(Si Myoung Lee) 박순기(Soon Ki Park) 류태훈(Tae-Hun Ryu)
• 간행물 정보: 『한국육종학회지』Vol.46 No.4, 389~399쪽, 전체 11쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2014.12.30

영문 초록

Genetically modified (GM) crops have been developed worldwide through the recombinant DNA technology and commercialized by various agricultural biotechnology companies. Commercialization of GM crops will be required the assessment of risk associated with the release of GM crops. The purpose of this research is a molecular characterization of introduced T-DNA in transgenic rice T4∼T6 generation lines harboring a pepper MsrB2 gene under the control of stress inducible Rab21 promoter, as a part of biosafety evaluation for drought-tolerant transgenic rice (Agb0103). We identified the structure and sequence of transformation vector of T-DNA and analyzed insertion sites, flanking sequences, and generational stability of inserted T-DNA in transgenic rice lines. The transformation vector was consisted of right border, a drought-tolerant CaMsrB2 gene unit (Rab21 promoter:CaMsrB2:PinII terminator), a selectable marker herbicide resistance unit (CaMV 35S promoter:bar:Nos terminator), and left border in sequential order. Based on the adaptor-ligation PCR and whole genome sequence database, we confirmed that T-DNA was introduced 2 copies (head to head type) at the position of 2,471,957∼2,472,049 bp of chromosome No. 8. From the generational stability study, T-DNAs were stably inherited through the T4 to T6 generations, and also stable expression of bar gene from T-DNA was confirmed. It was also confirmed that the backbone DNA of transformation vector containing antibacterial gene was not present in Agb0103 rice genome. These results will be filed to biosafety assessment document of Agb0103.

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키워드

Biosafety Drought-tolerant Progeny study