인삼 유전자원 장기보존을 위한 종자 초저온보존 조건 구명

• 영문명: Response of Pre and Post Treatments for Cryopreservation of Korean Ginseng Seeds on Recovering Viability
• 발행기관: 한국육종학회
• 저자명: 라자메토브 쉐르조드(Sherzod Rajametov) 이영이(Young-Yi Lee) 김영창(Young-Chang Kim) 이석영(Sokyoung Lee) 이정윤(Jung-Yoon Yi) 전영아(Young-Ah Jeon) 성정숙(Jung-Sook Sung) 이기안(Gi-An Lee) 곽재균(Jae-Gyun Gwak)
• 간행물 정보: 『한국육종학회지』Vol.46 No.4, 408~416쪽, 전체 9쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2014.12.30

영문 초록

We investigated the effect of pre and post-cryopreservation treatment on the dehisced Ginseng (Panax ginseng C.A. Meyer) seeds germination rate. All seeds covered with endocarp and 96.3% of them were opened, initial moisture content (IMC) and germination rate were 55.9% and 84.0%, respectively. According to the pre and post-cryopreservation methods, highest seeds germination rate (GR) was observed in non-precooled seeds which recovered at 40°C, in which MC was 8-12% and desiccated at 15℃. However, by prolonged drying the seeds GR comparatively decreased under MC of below 2.2%, reaching below rate 4% regardless of pre and post- cryo treatments, whereas the seeds dried at 25℃ airflow cabinet saved significantly high rate. The effects of desiccation and of cryopreservation on the survival and developmental pattern of roots and stems showed that the earliest appearance of the root in control seeds was detected after 10 days of bedding in GA medium, and the stem started to develop later on the third week of germination test. And, on the 30th day of the investigation stem growth rate significantly increased and showed equivalent data with root rate. Almost the same pattern showed the seeds after cryopreservation, but it significantly delayed in the development compared to control, where term of the investigation continued till 40 day than control 30 day. Additionally, non-precooling seeds in both desiccation and recovery methods were showed high root and stem growth compared to precooled treatments.

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REFERENCES

키워드

Seed Cryopreservation Precooling Recovery