생쥐의 뇌 발현에 관여하는 5 -UTR 도서관의 제조

• 영문명: Construction of 5 -Upstream Region Library Controlling Brain Expression in Mouse
• 발행기관: 한국산업기술융합학회(구. 산업기술교육훈련학회)
• 저자명: 장재선(Jae-Seon Jang)
• 간행물 정보: 『산업기술연구논문지』산업기술교육훈련논문지 제17권 2호, 63~69쪽, 전체 7쪽
• 주제분류: 공학 > 공학일반
• 파일형태: PDF
• 발행일자: 2012.06.30

영문 초록

We are constructed 5 -upstream region(UTR) library using mRNAs isolated from mouse brain. To exclude mRNA pool with the damage in the 5 -ends most of which don t include 5 -UTR, the mRNA pool is treated with bacterial alkaline phosphatase and tobacco acid pyrophosphstase. The two enzymes make the intact mRNAs have a phosphate group in the 5 -ends, while the damaged mRNAs possess hydroxy group in the 5 -ends of them. Consequently, only intact mRNAs including 5 -UTRs can attach a XB RNA linker to 5 -ends of them. First-stranded cDNAs synthesized by using ATG primers are amplifed from PCR with the ATG primers and the XB primers. The amplified PCR products, which contain 5 -UTR, are subjected to cloning to lambda DNA vector. The titer of constructed 5 -UTR library is 2.75 x 105 pfu/mL. To investigate the efficiency of the library, twenty eight clones are chosen at random and sequenced. By analysis of their sequences, it s efficiency is approximately 40% and the average size of 5 -UTRs is 132 bp, implying that the quality of the library is as good as it can be used to isolate promoters for genes expressed in moue brain.

목차

Ⅰ. 서 론

Ⅱ. 실험장치 및 방법

Ⅲ. 실험결과 및 고찰

Ⅳ. 결론 및 참고문헌

키워드

UTR Library Brain-Specific(뇌 특이적) Promoter(프로모터) Mouse(생쥐)