황련해독탕 중 3종 생리활성 물질의 HPLC-DAD 동시 정량분석법 확립

• 영문명: Simultaneous Analysis of three Marker Components in Hwangryunhaedok-tang by HPLC-DAD
• 발행기관: 대한약학회
• 저자명: 양혜진(Hye Jin Yang) 원진배(Jin Bae Weon) 마진열(Jin Yeul Ma) 마충제(Choong Je Ma)
• 간행물 정보: 『약학회지』제55권 제1호 (2011년), 64~68쪽, 전체 5쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2011.02.28

영문 초록

In this study, a high performance liquid chromatography-diode array detector method was established, for simultaneous determination of three compounds, berberine, palmatine and geniposide in Hwangryunhaedok-tang, To develop and validate method, C18 column (5 μm, 4.6 mm×250 mm) was used with gradient mobile phase, water containing 0.1% trifluoroacetic acid (TFA) and MeOH at the column temperature of 30oC. UV wavelength was set at 230 and 280 nm. Validation of the chromatography method was evaluated by linearity, precision and accuracy test. Calibration curve of standard components showed good linearity (R2>0.9999). The limits of detection (LOD) and limits of quantification (LOQ) varied from 0.05 to 0.17 μg/ml and 0.15 to 0.53 μg/ml, respectively. The relative standard deviations (RSDs) data of intra-day and inter-day test were in less than 2.99% and 1.90%, respectively. The results of the accuracy test were in the range of 98.36 to 102.52% with RSDs values 0.32 to 1.98%. The results of validation indicated that this method was a very accurate and sensitive assay.

키워드

Hwangryunhaedok-tang HPLC-DAD simultaneous determination validation