인간 HER-2 재조합 단백질을 사용한 항 HER-2 항체 단백질의 ELISA 정량 방법 개발

• 영문명: Development of a Quantitative ELISA for Anti HER-2 Antibodies using Human HER-2 Recombinant Proteins
• 발행기관: 대한약학회
• 저자명: 정선기(Sun Ki Jung) 류창선(Chang Seon Ryu) 정규진(Kyu Jin Choung) 송규용(Gyu Yong Song) 김상겸(Sang Kyum Kim)
• 간행물 정보: 『약학회지』제55권 제1호 (2011년), 16~21쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2011.02.28

영문 초록

HER-2 (Human Epidermal Growth Factor Receptor-2) is a protein giving higher aggressiveness in human breast cancers. Trastuzumab is a monoclonal antibody that targets HER-2 and is known to extend survival across all stages of HER2-positive breast cancer. In this study, we attempted to development of a quantitative ELISA (Enzyme-Linked ImmunoSorbent Assay) for evaluating anti HER-2 antibodies using human HER-2 recombinant proteins to support antibody producing processes and pharmacokinetic studies. We established direct or indirect ELISA method for the trastuzumab-like protein combined human recombinant HER-2. The ELISA method will prove to be great value in quantitating anti-HER-2 antibodies levels for developing anticancer antibodies.

키워드

ELISA Trastuzumab HER-2 breast cancer