An epidemiological comparative study on diagnosis of rodent leptospirosis in Mazandaran Province, northern Iran

• 발행기관: 한국역학회
• 저자명: Behzad Esfandiari Mohammad Reza Pourshafie Mohammad Mehdi Gouya Pejvak Khaki Ehsan Mostafavi Jamshid Darvish Soheila Moradi Bidhendi Hamed Hanifi Hossein Nahrevanian
• 간행물 정보: 『Epidemiology and Health』37, 1~9쪽, 전체 8쪽
• 주제분류: 의약학 > 면역학
• 파일형태: PDF
• 발행일자: 2015.05.30

영문 초록

OBJECTIVES: Leptospirosis is a zoonosis caused by leptospires, in which transmission occurs through contact with contaminated biological fluids from infected animals. Rodents can act as a source of infection for humans and animals. The disease has a global distribution, mainly in humid, tropical and sub-tropical regions. The aim of this study was to compare culture assays, the microscopic agglutination test (MAT), polymerase chain reac-tion (PCR), and nested PCR (n-PCR), for the diagnosis of leptospirosis in rodents in Mazandaran Province, northern Iran. METHODS: One hundred fifty-one rodents were trapped alive at 10 locations, and their urine and kidney samples were collected and used for the isolation of live Leptospira. The infecting serovars were identified and the antibody titres were measured by MAT, using a panel of 20 strains of live Leptospira species as antigens. The presence of leptospiral DNA was evaluated in urine and kidney samples using PCR and n-PCR. RESULTS: No live leptospires were isolated from the kidney and urine samples of the rodents. Different de-tection rates of leptospirosis were observed with MAT (21.2%), PCR (11.3%), and n-PCR (3.3%). The domi-nant strain was Leptospira serjoehardjo (34.4%, p= 0.28), although other serotypes were also found. The prev-alence of positive leptospirosis tests in rodents was 15.9, 2.6, and 2.6% among Rattus norvegicus, R. rattus, and Apodemus sylvaticus, respectively. CONCLUSIONS: Leptospirosis was prevalent in rodents in Mazandaran Province, northern Iran. MAT was able to detect leptospires more frequently than culture or PCR. The kidney was a more suitable site for identi-fying leptospiral DNA by n-PCR than urine. Culture was not found to be an appropriate technique for clinical diagnosis.

목차

Abstract

INTRODUCTION

MATERIALS AND METHODS

RESULTS

DISCUSSION

키워드

Culture Iran Leptospira Microscopic agglutination test Mazandaran Nested polymerase chain reaction Rodent