실험적 자가면역 포도막염 마우스 모델에서 Nod양 수용체 전사체의 안내 발현 양상 분석

• 영문명: Transcriptional Analysis of Nod-Like Receptors in a Mouse Model of Experimental Autoimmune Uveitis
• 발행기관: 대한안과학회
• 저자명: 김형민 김태완 정현정 류진숙 김미금 위원량 오주연,Hyeong Min Kim, MD, Tae Wan Kim, MD, PhD, Hyun Jeong Jeong, Jin Suk Ryu,Mee Kum Kim, MD, PhD, Won Ryang Wee, MD, PhD, Joo Youn Oh, MD, PhD,
• 간행물 정보: 『대한안과학회지』Ophthalmological Society,volume56,number1, 99~103쪽, 전체 5쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2015.01.15

영문 초록

Purpose: To evaluate the transcription pattern of Nod-like receptors (NLRs), the intracellular sensors, to detect danger signals in murine eyes with experimental autoimmune uveitis (EAU). Methods: EAU was induced in B6 (C57BL/6) mice by subcutaneous injection of human interphotoreceptor retinoid binding protein and intraperitoneal injection of pertussis toxin. At 1, 2, and 3 weeks post-immunization, the eyeballs were extracted and subjected to histological and molecular assays using real-time reverse transcription polymerase chain reaction. Results: The levels of nucleotide-binding oligomerization domain, Leucine rich Repeat and Pyrin domain 1 (NLRP1), NLRP3, nucleotide- binding oligomerization domain-containing protein 1 (NOD1), and NOD2 transcripts were increased at 2 weeks and gradually reduced thereafter. Notably, NLRP3 showed the highest expression in the eyes with EAU. Similarly, the transcript level of pro-inflammatory cytokine, interleukin-1β, increased and reached a peak at 2 weeks post-immunization. The retinal structure was severely damaged by inflammation at 3 weeks post-immunization. Conclusions: Among NLRs, NLRP3 may induce inflammation in eyes after EAU immunization. J Korean Ophthalmol Soc 2015;56(1):99-103

키워드

Experimental autoimmune uveitis, NLRP3, Nod-like receptors