배양된 망막신경절세포주에서 허혈전처치와 Protein kinase C의 역할

• 영문명: Ischemic Preconditioning and the Role of Protein Kinase C in Cultured Retinal Ganglion Cell Line
• 발행기관: 대한안과학회
• 저자명: 나경두 강성용 성공제 홍사민 전미진 김찬윤,Kyoung Doo Na, M.D., Sung Yong Kang, M.D., Gong Je Seong, M.D., Ph.D., Samin Hong, M.D., Mi-Jin Chun, Ph.D., Chan Yun Kim, M.D., Ph.D.
• 간행물 정보: 『대한안과학회지』Ophthalmological Society,volume49,number6, 979~986쪽, 전체 8쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2008.06.15

영문 초록

Purpose: To investigate the cellular protective effects of hypoxic preconditioning against oxidative stress in a staurosporine-differentiated RGC-5 cell line and the relevance of protein kinase C subtype expression. Methods: The minimum staurosporine concentration and exposure time necessary to morphologically fully differentiate RGC-5 cells were determined. Cytotoxic injury was provided by oxidative stress with 800 μM hydrogen peroxide (H2O2) for 15 hours to morphologically fully-differentiated cells. The cytoprotective effect of hypoxic preconditioning was found by exposing the cell line to 0.3% oxygen for different periods of time. Quantifiable changes in the expression of mRNAs and proteins of the isoenzymes α, β, γ, δ, ε, ζ of protein kinase C were determined before and after 1, 2, 15, and 24 hours of hypoxic preconditioning. Results: Axonal growth in RGC-5 cells after the induction of differentiation with staurosporine caused these cells to resemble neurons. The minimal concentration and exposure time to staurosporine that evoked full differentiation of RGC-5 cells was exposure to 2 μM staurosporine for 1 hour. An LDH assay demonstrated that hypoxic preconditioning had neuroprotective effects against hydrogen peroxide-induced oxidative stress. Protein and mRNA levels of PKC isoforms α and ε increased after preconditioning. Conclusions: Hypoxic preconditioning of staurosporine-differentiated RGC-5 cells had a cytoprotective effect against oxidative stress. The associated increase of mRNA and proteins of PKC isoenzymes α and ε suggest some functional relevance of these isoenzymes to the cytoprotective effects conferred by hypoxic preconditioning. J Korean Ophthalmol Soc 49(6):979-986, 2008

키워드

Ischemic preconditioning, Oxidative stress, Protein kinase C, RGC-5