은행잎추출물이 배양된 사람 섬유주세포의 생존에 미치는 영향

• 영문명: Effect of Ginkgo Biloba Extract on the Survival of Cultured Human Trabecular Meshwork Cells
• 발행기관: 대한안과학회
• 저자명: 김성재 강현구 김재우,Seong Jae Kim, M.D., Hyun Ku Kang, M.D., Jae Woo Kim, M.D.,
• 간행물 정보: 『대한안과학회지』Ophthalmological Society,volume47,number4, 643~648쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2006.04.30

영문 초록

Purpose: To investigate the effect of Ginkgo biloba extract (GBE, EGb-761) on the survival of cultured human trabecular meshwork cells (HTMC). Methods: Free radical scavenging activity of GBE was assessed with a DPPH assay. Primarily cultured HTMC were exposed to 10 and 100 ?g/ml of GBE. 0.3 mM sodium cyanide and 100 ?M hydrogen peroxide were added to the culture medium with GBE for 48 hr. Cellular survival and nitrite production were assessed by MTT assay and Griess assay, respectively. Results: GBE showed free radical scavenging activity. GBE increased the cellular survival of HTMC significantly in a dose-dependent manner under hypoxia or serum-deprived condition, but not to hydrogen peroxide. GBE increased nitric oxide production but not to statistically significant levels. Conclusions: GBE promotes proliferation and has cytoprotective effects in the context of HTMC exposed to serum-deprived or hypoxic conditions. However oxidative stress induced by hydrogen peroxide did not have an effect on proliferation of HTMC. In addition these effects were not related to the production of nitric oxide.

키워드

Ginkgo biloba extract, Hypoxia, Nitric oxide, Oxidative stress, Trabecular meshwork cells