가토 각막에서 자가 윤부이식 시 성공을 위한 최소 윤부 양에 대한 연구

• 영문명: Minimal Requirement of Limbal Epithelium for Successful Limbal Cell Transplantation in Rabbit Corneas
• 발행기관: 대한안과학회
• 저자명: 양유리 최남영 김진형 김만수,Yoo Ri Yang M.D., Nam Young Choi M.D., Jin Hyung Kim M.D., Man Soo Kim M.D.
• 간행물 정보: 『대한안과학회지』Ophthalmological Society,volume47,number4, 613~620쪽, 전체 8쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2006.04.30

영문 초록

Purpose: To investigate the minimal requirements of the limbal epithelium for successful limbal stem cell transplantation and the healing process. Methods: Nine rabbits were divided into 4, 6, and 8 clock-hour transplantation groups. Limbal autografts from the healthy fellow eye were transplanted to the iatrogenic damaged eye. The amniotic membrane served as a stem cell niche. Experimental corneas were evaluated by slit lamp examination and immunohistochemistry. Results: In the over 9 hours transplantation group, the healing process of the epithelium from the limbal stem cell was revealed and cornea-specific keratin k3, transcription factor p63, and connexin 43 were detected by immunohistochemistry. The normal corneal epithelium was regenerated after 60 days postoperatively in the fellow donor eye. Conclusions: Limbal cell transplantation of over 9 hours seems to be a safe and effective method in the treatment of severe ocular surface disorders. In addition, the donation of limbal epithelium for up to 8 hours did not affect the normal corneal regenerating capability.

키워드

Corneal epithelial stem cell, Limbal cell transplantation