결장암에 대한 활성 자연살해세포의 항암효능

• 영문명: Anticancer Effect of Activated Natural Killer Cells on Human Colorectal Tumor
• 발행기관: 대한약학회
• 저자명: 성혜란(Hyeran Sung) 김지연(Jee Youn Kim) 박민경(Min Gyeong Park) 김일회(Il-Hoi Kim) 이동욱(Dong Wook Lee) 한상배(Sang-Bae Han) 이종길(Chong-Kil Lee) 송석길(Sukgil Song)
• 간행물 정보: 『약학회지』제54권 제3호 (2010년), 192~199쪽, 전체 8쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2010.06.30

영문 초록

Colorectal cancer is one of the most common alimentary malignancies. In this study, the antitumor activity of activated human natural killer (NK) cells against human colorectal cancer was evaluated in vivo. Human NK cells are the key contributors of innate immune response and the effective functions of these cells are enhanced by cytokines. Human peripheral blood mononuclear cells (PBMC) were cultured with interleukin-2 (IL-2)-containing medium for 14 days and resulted in enriched NK cell population. The resulting populations of the cells comprised 7% CD3+CD4+ cells, 25% CD3+CD8+ cells, 13% CD3-CD8+ cells, 4% CD3+CD16/CD56+ cells, 39% CD3+CD16/CD56- cells, and 52% CD3-CD16/CD56+ cells. Tumor necrosis factor alpha (TNF-α), interferon gamma (IFN-γ), IL-2, IL-4, and IL-5 transcripts of the activated NK cells were confirmed by RT-PCR. In addition, activated NK cells at doses of 2.5, 5 and 10 million cells per mouse inhibited 10%, 34% and 47% of SW620-induced tumor growth in nude mouse xenograft assays, respectively. This study suggests that NK cell-based immunotherapy may be used as an adoptive immunotherapy for colorectal cancer patients.

키워드

natural killer cells adoptive immunotherapy SW620 colorectal cancer