HPLC-DAD-ion trap-MS를 이용한 인동 생리활성 물질의 동시분석

• 영문명: Simultaneous Analysis of Bioactive Metabolites from Caulis Lonicera japonica by HPLC-DAD-ion trap-MS
• 발행기관: 대한약학회
• 저자명: 유성광(Sungkwang Ryu) 원태형(Tae Hyung Won) 강삼식(Sam Sik Kang) 신종헌(Jongheon Shin)
• 간행물 정보: 『약학회지』제54권 제3호 (2010년), 157~163쪽, 전체 7쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2010.06.30

영문 초록

A high-performance liquid chromatography (HPLC) with DAD detector and electrospray ionization mass spectrometry (ESI-MS) was established for the simultaneous determination of coniferin (1), loganic acid (2), demethylsecologanol (3), sweroside (4) and loganin (5) from caulis Lonicera joponica. The optimal chromatographic conditions were obtained on an ODS column (5 μm, 4.6×150 mm) with the column temperature 35oC. The mobile phase was composed of (A) water with 0.1% formic acid and (B) methanol with 0.1% formic acid using a gradient elution, the flow rate was 0.3 ml/min. Detection wavelength was set at 254 nm. All calibration curves showed good linear regression (r2>0.998) within test ranges. The developed method provided satisfactory precision and accuracy with overall intra-day and interday variations of 0.16~3.28% and 0.14~1.99%, respectively, and the overall recoveries of 99.39~105.89% for the five compounds analyzed. The verified method was successfully applied to quantitative determination of the two types (phenolic compounds and iridoids) of bioactive compounds in 24 commercial caulis L. japonica samples from different markets in Korea and China. The analytical results demonstrated that the contents of the five analytes vary significantly with sources.

키워드

HPLC-DAD-ion trap-MS caulis Lonicera japonica iridoids phenolic compounds