갈근탕 추출물의 카드뮴 독성에 대한 세포 보호효과

• 영문명: Cytoprotective Effect of Galgeuntang Extract on Cadmium-Induced Cytotoxicity
• 발행기관: 대한약학회
• 저자명: 박소윤(So Yun Park) 백종민(Jong Min Baek) 백승화(Seung Hwa Baek)
• 간행물 정보: 『약학회지』제54권 제3호 (2010년), 151~156쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2010.06.30

영문 초록

Cytotoxicity of cadmium on NIH 3T3 fibroblasts was utilized in order to discover antitoxic compound in Galgeuntang extract in this study. Treatment groups were chosen as follows; control (medium only), MTT50 group and five experimental groups. MTT {3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazoliumbromide} method was performed to evaluate the cytotoxicity of cell organelles and IC50 was also measured. Accordingly we have examined the detoxification effects of Galgeuntang extract on cadmium-treated NIH 3T3 fibroblasts to observe morphological changes by the light microscopy. Galgeuntang extract showed cytoprotective effects on cadmium-induced cytotoxicity. Furthermore, Galgeuntang showed a dose-dependency in detoxication. The phenolic content of Galgeuntang ethanol extract was higher than that of water content. These results suggest that Galgeuntang extract may be used as a cytoprotective agent against cadmium (II)-mediated cytotoxicity.

키워드

cytoprotective effect cadmium (Ⅱ)-mediated cytotoxicity NIH 3T3 fibroblasts Galgeuntang extract