Maackia fauriei 유래 렉틴의 중성당 및 아미노당 조성

• 영문명: Neutral and Amino Sugars Composition of a Lectin from Maackia fauriei
• 발행기관: 대한약학회
• 저자명: 나광흠(Kwang Heum Na) 박병태(Byung Tae Park) 박재완(Jae Wan Park) 한경진(Kyong Jin Han) 박현주(Hyun Joo Park) 김하형(HaHyung Kim)
• 간행물 정보: 『약학회지』제53권 제1호 (2009년), 34~40쪽, 전체 7쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2009.02.28

영문 초록

The glycosylation of therapeutic glycoproteins can affect their efficacy, stability, solubility, and half-life. Analyzing the composition of monosaccharides, such as that of neutral and amino sugars, is the first step for elucidating the structure of glycan attached to glycoproteins. In the present study, neutral and amino sugars of lectin obtained from Maackia faurieiwere analyzed using an enzyme-linked lectinsorbent assay (ELLA) and high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD). Peroxidase-labeled lectins such as concanavalin A, Ricinus communis agglutinin, and soybean agglutinin were used for ELLA, since they specifically bind to the monosaccharide residue most frequently encountered in a glycan. The hydrosylate of lectin was prepared by treatment with trifluoroacetic acid, which resulted in the lectin mainly possessing the N-glycan consisting of 98.1 pmol Fuc, 342.1 pmol GlcN, 51.9 pmol Gal, 678.9 pmol Man, and 330.7 pmol Xyl. The present results demonstrate that ELLA and HPAEC-PAD are very effective methods for rapidly estimating the types and relative amounts of monosaccharides in intact glycoproteins.

키워드

glycoprotein enzyme-linked lectinsorbent assay HPAEC-PAD monosaccharide