어성초의 카드뮴에 대한 독성억제효과(II)

• 영문명: The Inhibitory Effects of Houttuynia cordata THUNB against Cadmium induced Cytotoxicity (II)
• 발행기관: 대한약학회
• 저자명: 이정호(Jeong Ho Lee) 유일수(Il Soo You) 김종수(Jong Soo Kim) 이기남(Ki Nam Lee) 정우영(Woo Young Chung) 한두석(Du Seok Han) 백승화(Seung Hwa Baek)
• 간행물 정보: 『약학회지』제44권 제5호 (2000년), 432~439쪽, 전체 8쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2000.10.31

영문 초록

This study was conducted to investigate the antitoxic component in aqueous extract of Houttuynia cordata THUNB. The results were as follows: Generally, detoxification effects by Houttuynia cordata THUNB extract increased in proportion to the extract concentrations in rats. When 40mg/kg dosage of Houttuynia cordata THUNB extract was administrated, Houttuynia cordata THUNB extract showed the highest antitoxic effects in metallothionein induction. After the extract treatment, body weights increased in proportion to the extract concentrations. However, after 3 weeks, the body weight decreased insignificantly. From the above results, Houttuynia cordata THUNB extract increased metallothionein concentration and decreased the toxicity of cadmium in rats. In vitro the antitoxic activity of aqueous extract of Houttuynia cordata THUNB on NIH 3T3 fibroblasts was evaluated by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazoliumbromide) and SRB (sulforhodamine B protein) assays. The light microscopic study was carried out to observe morphological changes of the treated cells. These results were obtained as follows; The concentration of 10-2mg/ml of Houttuynia cordata THUNB extract was shown significant antitoxic activity. The number of NIH 3T3 fibroblasts were increased and. tend to regenerate. These results suggest that Houttuynia cordata THUNB extract retains a potential antitoxic activity.

키워드

Houttuynia cordata THUNB Antitoxic agent Metallothionein MTT assay SRB assay.