S. abortus 유래 LPS와 E. coli 유래 LPS에 의한 패혈증성 쇽 유도 작용 비교

• 영문명: Differential Induction of Scptic Shock by Lipopolyscchrides from E. coli and S. abortus
• 발행기관: 대한약학회
• 저자명: 조재열(Jae Youl Cho) 유은숙(Eun Sook Yoo)
• 간행물 정보: 『약학회지』제51권 제1호 (2007년), 44~50쪽, 전체 7쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2007.02.28

영문 초록

Acute septic shock is one of inflammatory diseases mediated by pro-inflammatory cytokines such as tumor necrosis factor (TNF)-α. In this study, we examined the pathological difference and mechanism of lipopolysaccharides isolated from E. coli (E-LPS) or S. abortus (S-LPS) on inducing acute septic shock in ICR mouse. All mice were died by intraperitoneal treatment of S-LPS with 0.75 mg/kg, whereas E-LPS treated with even 3 mg/kg only showed 30% of mice lethal, indicating that S-LPS may be more feasible in triggering a strong septic shock condition. The secretion pattern of TNF-α, a critical pro-inflammatory cytokine in septic shock condition, was also distinct between E-LPS-and S-LPS-treated groups. Thus, S-LPS strikingly increased serum level of TNF-α (6 ng/ml) at 1 h, while E-LPS just displayed at 2 ng/ml level. However, the interaction of S-LPS with LPS receptor, toll like receptor (TLR)-4 was not stronger than that of E-LPS, according to experiments with macrophage cell line RAW264.7 cells. Thus, E-LPS rather than S-LPS strongly enhanced the production of TNF-α. Interestingly, S-LPS more strongly up-regulated splenocyte proliferation, compared to E-LPS group, whereas there was no difference between S- or E-LPS treated groups in proliferation of Balb/c- or c57BL/6-originated splenic lymphocytes. Therefore, our data suggest that S-LPS is a more active endotoxin and that the strong septic shock-inducing effect of S-LPS seems due to the enhanccment of early TNF-α production and S-LPS sensitive lymphocyte proliferation.

키워드

Septic shock LPS type TNF-α NO splenic lymphocyte proliferation