췌장 베타세포에서 인터루킨-1beta로 유도한 인슐린 의존형 당뇨병 실험 모델

• 영문명: Prediabetic In vitro Model in Pancreatic Beta Cells Induced by Interleukin-1beta
• 발행기관: 대한약학회
• 저자명: 이인순(Ihn Soon Lee) 이인자(In Ja Rhee) 김경태(Kyong Tai Kim)
• 간행물 정보: 『약학회지』제42권 제4호 (1998년), 408~413쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1998.08.31

영문 초록

To establish prediabetes in vitro/ model concerning the etiology of Insulin Dependent Diabetes Mellitus (IDDM) in cellular level we have designed experimental prediabefic model in pancreatic beta cells. RINm5F, HIT-T15 and isolated rat islets were chosen as pancreatic beta cells. Since interleukin-1beta-induced beta cell cytotoxicity has been implicated in the autoimmune cytotoxicity of IDDM, we used inteleukin-1beta as diabetogenic agent. For establishment of prediabetic in vitro model, the degree of beta cell deterioration was determined by cell proliferation, insulin release and morphological appearance. Cell proliferation, insulin release and morphology were changed dose-dependently in condition that inteleuldn-1beta was exposured to pancreatic beta cells. The concentration and exposure time of interleukin-1beta to set up prediabetic model in beta cell lines and isolated rat islets were 100-1000U/ml, 48hr. And 25-100U/ml, 48hr, respectively.

키워드

IDDM(Insulin Dependent Diabetes Mellitus) interleukin-1β RINm5F HIT-T15 rat islets insulin cell proliferation prediabetic model.