토끼 위체에서 비-아드레날린 비-콜린성 이완반응의 하행성 감소

• 영문명: Downward Decrease of Non-adrenergic Non-cholinergic Relaxation in the Rabbit Gastric Body
• 발행기관: 대한약학회
• 저자명: 홍은주(Eun Ju Hong) 최지은(Ji Eun Choi) 박미선(Mi Sun Park) 김명우(Myung Woo Kim) 최수경(Su Kyung Choi) 홍승철(Sung Cheul Hong)
• 간행물 정보: 『약학회지』제41권 제3호 (1997년), 389~398쪽, 전체 10쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1997.06.30

영문 초록

Non-adenergic non-cholinergic (NANC) innervation on the circular muscle of the rabbit gastric body was investigated by observing the magnitudy of relaxations induced by the electrical field stimulation (EFS). Strips were cut from the greater curvature of the gastric body and stimulated with 5s trains of 0.5 ms pulses at 1-20 Hz, 40 V. The EFS induced transient frequency-dependent contractons, followed by a slowly recovering relaxation ewpecially at higher frequency of the EFS. In the presence of atropine and guanethidine, the contractions were virtually abolished, while the frequency-dependent relaxations by the EFS remained unaffected. The magnitude of relaxations progressively decreased as the location of the strips gets closer to the bottom of the gastric body. The relaxations were ablished by tetrodotoxin, indicating that their orgin is the NANC nerve stimulation. NG-nitro-L-arginine (L-NNA, 10-100 mcM), the inhibitor of nitric oxide (NO)-synthase, caused a concentration-dependent inhibition of the NANC relaxations. The inhibitory effects of L-NNA were not affected gy the location of the strips and were reversed by L-arginine, the precursor of NO-biosynthesis. Hemoglobin (20-60 mcM), a NO scavenger, inhibited the NANC relaxation s in a concentration-dependent manner. This inhibition was more prominent in the NANC relaxations observed in the lower portion of the gastric body and the relaxations induced ly lower frequencies of the EFS. Methyelne blue (10-100 mcM), an inhibitor of cytosolic guanylate cyclase, markedly inhibited the NANC relaxations, almost abolishing the response at a higher dose (100mcM). These results suggest that NANX innervation of the rabbit gastric body progeressively decrease as he location of the strips gets closer to the bottom of the gastric body, and that the NANC relaxation is primarily mediated by NO-guanosine 3'',5''-cyclic monophophate (cyclic GMP).

키워드

Rabbit gastric body Electrical field stimulation Inhibitory NANC innervation NO-cyclic GMP pathway.