재조합 인간 상피세포성장인자(DWP401)의 흰쥐에서의 in vivo와 in vitro 대사

• 영문명: in vivo and in vitro Metabolism of Recombinant Human Epidermal Growth Factor (DWP401) in Rats
• 발행기관: 대한약학회
• 저자명: 고여욱(Yeo Wook Koh) 남권호(Kouen Ho Nam) 정주영(Ju Young Jung) 박승국(Seung Kook Park) 유영효(Young Hyo Yu) 김재환(Jae Hwan Kim) 한건(Kun Han) 박명환(Myung Hwan Park) 심창구(Chang Koo Shim)
• 간행물 정보: 『약학회지』제41권 제3호 (1997년), 381~388쪽, 전체 8쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1997.06.30

영문 초록

Metabolism of DWP401, recombinant juman epidermal growth factor, was examined in vivo and in vitro in rats. When 125I-labeled DWP401 was administered at a dose of 50 mcg/kg by i.v. injection. 125I-labeled DWP401 was rapidly degraded within 30 minytes above 93%. Thin layer chromatography analysis of urine collected for 24 hr after i.v. administration of 125I-labeled DWP401 showed ohly one spot on a X-ray film which was considered as diiodo-tyrosine. This result suggests tha 125I-labeled DWP401 was completely digested into free amino acids without any specific intermediate polypeptides. About 42.1% of the administered iodine was recovered in 24 hr. For in vitro degradation study, 125I-labeled DWP401 was added to plama and tissue homogenates of rats and incubated at 37oC. Almost 98% of the added radioactivity recovered from the protein fraction of the liver, kidey, small intestine, stomach and spleen decreased rapidly. For examplem the recovery rates of 125I-labeled DWP401 were 58.6, 63.2, 39.9, 52.9 and 66.8% after 4hrs of incubation in respective organ homogenates.

키워드

DWP401 recombinant human epidermal growth factor metabolism rat