32P-Postlabelling 방법을 이용한 미토마이신 C에 의하여 형성된 DNA adduct의 검출

• 영문명: Detection of DNA Adduct Formed by Mitomycin C by 32P-Postlabelling
• 발행기관: 대한약학회
• 저자명: 정혜윤(He Yun Jeong) 김재현(Jae Hyun Kim) 박창원(Chang Won Park) 이동권(Dong Kwon Rhee)
• 간행물 정보: 『약학회지』제40권 제4호 (1996년), 442~448쪽, 전체 7쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1996.08.31

영문 초록

Mitomycin C(MMC) has been used as an anticancer drug and behaves as an alkylating agent forming covalent cross-link between complementary strands of double strand DNA. The purpose of this research was to determine number of DNA adducts, formed in vivo by Mitomycin C, in mouse organs. DNAs from liver, lung, brain and pancreas were isolated and used for 32P-postlabelling. The labeled nucleotides were separated by 2D-TLC and subjected to autoradiography. Numbers of MMC-DNA adducts were 9,9,5,4 in liver, pancreas, lung and brain, respectively.

키워드

32P-Postlabelling DNA adduct mitomycin C.