HPLC를 이용한 고추엑스 제제 중 Capsaicin과 Dihydrocapsaicin의 분리정량

• 영문명: Differential determination of Capsaicin and Dihydrocapsaicin from Capsicum extract preparation by HPLC.
• 발행기관: 대한약학회
• 저자명: 엄동옥(Dong Ok Eom) 윤규섭(Kyu Sup Yoon)
• 간행물 정보: 『약학회지』제33권 제4호 (1989년), 241~245쪽, 전체 5쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1989.08.25

영문 초록

A new application of high performance liquid chromatography for the determination of capsaicin and dihydrocapsaicin in cataplasma preparation was investigated. Optimum conditions for a good separation and detection were determined; 0.93 volt, acetonitrile: H3PO4 sol (pH 2.8), sample size; 7mcl. Recovery rates of capsaicin and dihydrocapsaicin from mixed artificial preparations were 99.9% and 98.4% respectively. Also reproducibility tests showed that the coefficient of variation was 1.59% for capsaicin and 1.14% for dihydrocapsaicin. There was no interference with cataplasma preparation containing thymol, dl-camphor and the commonly encountered excipients or additives such as gelatine, glycerine. This method was rapid, accurate and it gave higher sensitivity than other method.

키워드

Capsacin dihydrocapsaicin cataplasma preparation.