해양칠게 혈림프로부터 렉틴의 분리 및 당 결합특성

• 영문명: Isolation and Carbohydrate Binding Specificity of a Lectin from the Hemolymph of Coastal Crab Macrophthalmus Japonicus
• 발행기관: 대한약학회
• 저자명: 김하나(Ha Na Kim) 정우혁(Woo Hyuk Chung) 배찬영(Chan Hyung Bae) 황광우(Kwang Woo Hwang) 김하형(HaHyung Kim)
• 간행물 정보: 『약학회지』제50권 제3호 (2006년), 166~171쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2006.06.30

영문 초록

A calcium-dependent sialic acid-binding lectin has been isolated by thyroglobulin-affinity chromatography from the coastal crab Macrophthalmus Japonicus. This lectin, Macrophthalmus Japonicus lectin (MJL), was eluted with 50mM Tris-HCl, 0.3M NaCl, 10mM EDTA, and the recovery yield from the crude protein extract was about 5.6%. The molecular weight of MJL was estimated as 65 kDa in SDS-PAGE both under reducing and non-reducing conditions. MJL induced an agglutination reaction in rabbit, rat, and mouse erythrocytes, but not in human ABO types. This activity was effectively inhibited by sialoglycoproteins such as fetuin, bovine submaxillary mucin, and thyroglobulin.

키워드

lectin crab sialoglycoprotein