착체 생성에 의한 황련 알칼로이드의 분리 및 정량

• 영문명: Isolation and Determination of Alkaloids in Coptis Rhizome by Forming Complex
• 발행기관: 대한약학회
• 저자명: 임소연(So Yun Lim) 김대근(Dae Keun Kim) 신태용(Tae Yong Shin) 임종필(Jong Pil Lim) 엄동옥(Dong Ok Eom)
• 간행물 정보: 『약학회지』제46권 제4호 (2002년), 226~230쪽, 전체 5쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2002.08.31

영문 초록

The Coptis Rhizome is known for containing a number of isoquinoline type alkaloids. Berberine, coptisine and palmatine are the major constituents of alkalolds. The alkaloids were isolated and determined by forming complex from Coptis japonica (Ranunculaceae). For the determination of these alkaloids, a new spectrophotometric method was developed with a simple and selective sample clean-up using thiocyanatocobaltate(II) complex ion. The absorbance of alkaloidal complex in 1,2- dichloroethane solution was measured at 625 nm. A calibration curve for the alkaloids isolated from Coatis Rhizome was linear over the concentration range of 0.2∼0.3 mg/ml. The method proved to be rapid, simple and reliable for the isolation and the determination of the alkaloids in Coatis Rhizome.

키워드

Coptis Rhizome alkaloids isolation determination spectrophotometric method.