소적혈구로부터 새로운 헝태의 세포질 포스포리팍제 A2의 동정 및 특성규명

• 영문명: Identification and Characterization of a Novel Cytosolic Phospholipase A2 from Bovine Red Blood Cells
• 발행기관: 대한약학회
• 저자명: 신혜숙(Hae Sook Shin) 김하동(Ha Dong Kim) 장동훈(Dong Hoon Chang) 전형준(Hyung Jun Jeon) 유충규(Chung Kyu Ryu) 정성윤(Sung Yun Jung) 김대경(Dae Kyong Kim)
• 간행물 정보: 『약학회지』제45권 제4호 (2001년), 407~412쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2001.08.31

영문 초록

A Ca2+_dependent PLA2 activity termed Rpla2, was detected in the cytosol of bovine red blood cells (RBCs). The Rplab was characterized as a similar form to Group IV Cpla2, but different in secteral column chromatographic profiles including an anion exchange column.73 examine whether this Rpla2 is different from the well characterized Rpla2, a quinone derivative, BJ5O, was developed and tested for the inhibitory effect on the two PLA2 enzymes. The rPLA2 activity was inhibited by a quinone derivative (BJ5O) with ID50 of 20μM., but had no effect on the cPLA2, suggesting that rPLA2 may be a novel type of cytosolic PLA2 in RBCs.

키워드

phospholipase A2 BJ50 arachidonyl trifluoromethyl ketone (AACOCF3).