해양동물 눈알고둥으로부터 새로운 렉틴 성분의 분리 및 정제

• 영문명: Purification and Charcterization of A New Lectin from Marne Animal Lunella coronata coreensis
• 발행기관: 대한약학회
• 저자명: 소명숙(Myung Suk So) 서영아(Young Ah Suh) 전경희(Kyung Hee Jeune) 정시련(See Ryun Chung)
• 간행물 정보: 『약학회지』제36권 제3호 (1992년), 241~249쪽, 전체 9쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1992.06.29

영문 초록

The whole body extract of Lunella coronata coreensis agglutinated nonspecifically human and other animal erythrocytes. A new lectin was purified by the following procedures: 0.15 M NaCl extraction, salt fractionation, gel filtration, anionic and cationic ion exchange column chromatographies. Through these purification procedures, specific activity of LCC-I was increased from 276 to 9714.3units/mg, And on polyacrylamide gel electrophoresis, LCC-I exhibited one major band. A molecular weight of LCC-I was assumed to be 20,000 by sodium dodesyl sulfate polyacrylamide gel electrophoresis. The purified lectin was relatively stable at various pH and heat. Among the tested sugars, lactose and lactulose inhibited lectin activity at a concentration of 6.25mM, respectively.