흰쥐의 분리 간세포에서 스트렙토조토신의 독성

• 영문명: Toxicity of Streptozotocin in Isolated Rat Hepatocytes
• 발행기관: 대한약학회
• 저자명: 박기숙(Ki Suk Park) 정정철(Jong Chol Chong) 문창규(Chang Kiu Moon) 정진호(Jin Ho Chung)
• 간행물 정보: 『약학회지』제36권 제1호 (1992년), 80~86쪽, 전체 7쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1992.02.28

영문 초록

Streptozotocin (STZ) is a naturally occurring nitrosoamide used extensively to produce diabetes in experimental animals. Our previous study has demonstrated that i.v. administration of streptozotocin induces significant red blood cell hemolmysis in rats. Since it has been reported that the highest concentration of STZ is found in the liver, the effect of STZ in freshly isolated rat hepatocytes has been investigated. STZ treatment (10mM) did not cause significant loss of viability throughout 4 hour incubation, while high dose of STZ-induced hyperglycemia in vivo did not affect its hepatotoxicity. To investigate the mechanism of the toxicity, intracellular total glutathione level was determined. Treatment with 10mM STZ which was not toxic to hepatocytes led to complete depletion of intracellular glutathione level within 1 hour incubation. These results suggest that STZ-induced hepatotoxicity may be independent on the intracellular glutathione depletion.