L-PHA 렉틴의 분리 정제 및 면역학적 연구

• 영문명: Purification and Immunochemical Studies on L-PHA Lectin
• 발행기관: 대한약학회
• 저자명: 정시련(See Ryun Chung) 서영아(Young Ah Suh) 소명숙(Myung Suk So) 전경희(Kyung Hee Jeune-Chung)
• 간행물 정보: 『약학회지』제28권 제3호 (1984년), 139~147쪽, 전체 9쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1984.06.30

영문 초록

L-PHA, a lectin having lymphoagglutinating activity but devoid of erythroagglutinability which is contained in Korean white kidney bean (Phaseolus vulgaris L.), was isolated and purified through several techniques such as ion exchange chromatography, hydroxyapatite column and affinity chromatography. Purified L-PHA was identified as a single band by polyacrylamide disc gel electrophoresis. The molecular weight of the L-PHA was estimated about 125,000 daltons by polyacrylamide gel electrophoresis and it was turned out having one subunit (probably dimer of the Yachnin''s model) of Mr~60,000. Immunochemical studies also tried and these results reconfirmed the purity of this L-PHA.