발효 황련해독탕의 생물 전환 성분분석

• 영문명: Analysis of Bioconversion Components of Fermentation Hwangryunhaedok-tang
• 발행기관: 대한약학회
• 저자명: 이광진 이보형 정필문 량춘 마진열
• 간행물 정보: 『약학회지』제57권 제4호 (2013년), 293~298쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2013.08.31

영문 초록

Hwangryunhaedok-tang (HRT) is a traditional herbal medicine, which has been known as a useful prescription for anti-biotic, anti-inflammatory, anti-oxidative and immunosuppressive activity. In this study, the variation in the amount of eight bioactive components of Hwangryunhaedok-tang (HRT) and its fermentation HRT with Lactobacillus casei KFRI 127, Lactobacillus curvatus KFRI 166 and Lactobacillus confuses KFRI 227 was investigated via high-performance liquid chromatography coupled with diode array detection (HPLC-DAD). Simultaneous qualitative and quantitative analysis of eight bioactive components; geniposide, genipin, baicalin, wogonoside, palmatine, berberine, baicalein and wogonin was achieved by comparing their retention times (tR) and UV spectra with those of the standard components. All calibration curve of standard components showed good linearity (r 2 >0.979). As a result, the geniposide amount was 15.52±0.19 µg/ mg that as a main components in HRT. The wogonoside was decreased by 29.28~58.35% with Lactobacillus casei KFRI 127 and L. confuses KFRI 227 (3.17±0.31 µg/mg and 3.55±0.13 µg/mg) compared with the original HRT (5.02±0.14 µg/mg). Otherwise wogonin was increased by 16.28~41.86% with Lactobacillus casei KFRI 127 and L. confuses KFRI 227 (0.61±0.01 µg/mg and 0.50±0.02 µg/mg) compared with the original HRT (0.43±0.00 µg/mg). HRT fermented with L. casei KFRI 127 and L. confuses KFRI 227 were evaluated as creating the changes in wogonoside to that aglycon wogonine. In the fermented HRT using Lactobacillus acidophilus KFRI 166, the genipin was only detected, among 3 species of fer- mentation strains. Thus, these results considered that the strains 166 were exhibited the remarkable changes in genipin.

키워드

Bioconversion Fermentation Hwangryunhaedok-tang Identification HPLC-DAD