HPLC를 이용한 정공등의 다성분 동시함량분석

• 영문명: Simultaneous Quantification Analysis of Multi-components on Erycibae Caulis by HPLC
• 발행기관: 대한약학회
• 저자명: 전혜진 유정 황완균
• 간행물 정보: 『약학회지』제57권 제4호 (2013년), 272~281쪽, 전체 10쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2013.08.31

영문 초록

In this study, we developed and validated the HPLC method using the isolated components from Erycibae cau- lis. Their structures were elucidated by spectroscopic methods including UV, 1 H-NMR, 13 C-NMR, FAB-Mass and ESI-Mass as Compound 1 (crypto-chlorogenic acid), Compound 2 (scopolin), Compound 3 (neochlorogenic acid) and Compound 4 (3,4- di-O-caffeoylquinic acid). Major three compounds and scopoletin were decided as resentative components of Erycibae caulis. We established HPLC analytical method by using the representative components and 20 commercial samples which were collected considering to various cultivated area. The HPLC fingerprinting was successfully achieved with an AKZO NOBEL Kromasil 100-5C18 column. The mobile phase consisted of 0.5% acetic acid in water (A) and methanol (B) using gradient method of 85(A) to 50(A) for 35min. The fingerprints of omatograms were recorded at an optimized wavelength of 330 nm. This developed analytical method was validated with specificity, selectivity, accuracy and precision. And it is sug- gested that scopolin, scopoletin, neochlorogenic acid, 3,4-di-O-caffeoylquinic acid were more than 0.162%, 0.133%, 0.057%, 0.044%, respectively. In addition, principal component analysis (PCA) was performed on the analytical data of 20 different Erycibae caulis samples in order to classify samples collected from different regions. We hope that this assay can be readily utilized as quality control method for Erycibae caulis.

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키워드

Erycibae caulis Erycibe obtusifolia components Convolvulaceae crypto-chlorogenic acid scopolin neochlo-rogenic acid 3,4-di-O-caffeoylquinic acid scopoletin HPLC PCA