생체에서 분리된 혈관조직에서 아데노바이러스벡터를 이용한 특정 단백질의 발현

• 영문명: Targeted Protein Expression in Freshly Isolated Vascular Tissues by Using Adenoviral Vector
• 발행기관: 대한약학회
• 저자명: 허양훈 김학림
• 간행물 정보: 『약학회지』제57권 제4호 (2013년), 265~271쪽, 전체 7쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2013.08.31

영문 초록

Treatments of vascular disease via modulating the expression of specific proteins by gene transfer have been attempted in various studies over the past few years. Among several methods to deliver genes, adenovirus currently has been used because of a number of positive aspects. In this study, we test adenoviral vector as a potential mediator in the treatment of vascular disease by using freshly isolated vascular tissues not cultured vascular cells. Freshly isolated vascular tissues were directly exposed to adenoviral vector pAd5CMVmcsIRESeGFPpA to check the possibility of GFP expression in different layer of vascular tissues. We found that the GFP expression by using adenoviral vector experiments is mainly focused on the adventitia and failed to detect GFP expression at endothelial layer or vascular smooth muscle layer in vas- cular tissues. However, we also found that several integrin receptors are robustly expressed in vascular smooth muscle, thus the limited expression of protein in vascular smooth muscle are not likely the lack of integrin receptors. In conclusion, adenovirus could not be a good tool for a specific protein expression in vascular smooth muscle cell. Thus, the application of adenovirus as a tool for gene therapy of vascular smooth muscle cells in clinical therapeutic trial need to be optimized further.

목차

실험방법

실험결과 및 고찰

결 론

감사의 말씀

참고문헌

키워드

adenovirus vector integrin vascular smooth muscle cell protein expression tissue culture