Profiling of endogenous metabolites and changes in intestinal microbiota distribution after GEN-001 (Lactococcus lactis) administration

• 발행기관: 대한생리학회-대한약리학회
• 저자명: Min-Gul Kim Suin Kim Ji-Young Jeon Seol Ju Moon Yong-Geun Kwak Joo Young Na SeungHwan Lee Kyung-Mi Park Hyo-Jin Kim Sang-Min Lee Seo-Yeon Choi Kwang-Hee Shin
• 간행물 정보: 『The Korean Journal of Physiology & Pharmacology』제28권 제2호, 153~164쪽, 전체 12쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 2024.03.01

국문 초록

This study aimed to identify metabolic biomarkers and investigate changes in intestinal microbiota in the feces of healthy participants following ad-ministration of Lactococcus lactis GEN-001. GEN-001 is a single-strain L. lactis strain isolated from the gut of a healthy human volunteer. The study was conducted as a parallel, randomized, phase 1, open design trial. Twenty healthy Korean males were divided into five groups according to the GEN-001 dosage and dietary control. Groups A, B, C, and D1 received 1, 3, 6, and 9 GEN-001 capsules (1 × 1011 colony form-ing units), respectively, without dietary adjustment, whereas group D2 received 9 GEN-001 capsules with dietary adjustment. All groups received a single dose. Fecal samples were collected 2 days before GEN-001 administration to 7 days after for untargeted metabolomics and gut microbial metagenomic analyses; blood samples were collected simultaneously for immunogenicity analysis. Levels of phenylalanine, tyrosine, cholic acid, deoxycholic acid, and tryptophan were significantly increased at 5–6 days after GEN-001 administration when compared with predose levels. Com-pared with predose, the relative abundance (%) of Parabacteroides and Alistipes sig-nificantly decreased, whereas that of Lactobacillus and Lactococcus increased; Lacto-bacillus and tryptophan levels were negatively correlated. A single administration of GEN-001 shifted the gut microbiota in healthy volunteers to a more balanced state as evidenced by an increased abundance of beneficial bacteria, including Lactobacillus, and higher levels of the metabolites that have immunogenic properties.

목차

INTRODUCTION

METHODS

RESULTS

DISCUSSION

키워드

Biological products Gastrointestinal microbiome Lactococcus lactis Metabolomics Microbiota