Antimicrobial polyhydroxybutyrate submicron fiber mat loaded with extract of Hypericum perforatum

• 영문명: Antimicrobial polyhydroxybutyrate submicron fiber mat loaded with extract of Hypericum perforatum
• 발행기관: (사)한국식물생명공학회
• 저자명: Milos Beran Ales Horna Viktor Vorisek Eliska Berkova Radka Korinkova Vojtech Trousil
• 간행물 정보: 『Journal of Plant Biotechnology』49권 3호, 257~270쪽, 전체 14쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2022.09.30

영문 초록

The aim of this work was to prepare a new biodegradable polyhydroxybutyrate (PHB) submicron fiber mat loaded with hypericin-rich Hypericum perforatum raw extract by centrifugal spinning technology, an alternative approach to the traditional method of electrospinning to fabricate nanofibers or microfibers from solutions at high speed and low cost. Hypericins in methanol/acetone extract of H. perforatum were determined by UHPLC-MS/MS and HPLC/PDA. Submicron fiber mats composed of pure PHB or PHB enriched with H. perforatum extract were prepared using a pilot plant demonstrator for the centrifugal spinning technology and characterized by SEM. Singlet oxygen production was quantified by the 1,3-diphenylisobenzofuran (DPIBF) method in hexane. The results proved a significant production of singlet oxygen by the prepared submicron fiber mat. We also found a significant antibacterial activity against the bacterial strain Escherichia coli CCM 5417 by a method in accordance with JIS Z 2801/ISO 22196 standards. The H. perforatum extract-enriched PHB submicron fiber mats showed potential for the development of self-cleaning and antimicrobial air filters.

목차

Introduction

Materials and Methods

Results and Discussion

Acknowledgement

References

키워드

Hypericum perforatum Hypericin extract Polyhydroxybutyrate Submicron fiber Singlet oxygen Antimicrobial air filter