Induction of in vitro root tubers in Holostemma annulare (Roxb.) K. Schum. for the production of bioactive metabolites

• 영문명: Induction of in vitro root tubers in Holostemma annulare (Roxb.) K. Schum. for the production of bioactive metabolites
• 발행기관: (사)한국식물생명공학회
• 저자명: Padmavathi Amma Somasekharan Nair Smitha Devi Achuthan Sudarsanan Hemanthakumar
• 간행물 정보: 『Journal of Plant Biotechnology』49권 3호, 230~239쪽, 전체 10쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2022.09.30

영문 초록

Holostemma annulare (Family Asclepiadaceae) is an invaluable vulnerable medicinal plant; the root tubers are used in Ayurveda medicine and by folk healers to treat various ailments. In this study, Schenk and Hildebrandt medium fortified with the cytokinins 6-benzyl adenine, kinetin, and auxins, including indole 3-butyric acid, indole 3-acetic acid, α-naphthaleneacetic acid, and 2,4-dichloro-phenoxyacetic acid, were checked for their efficiency on root tuber induction from different explants. Adventitious root tubers were more successfully induced from in vitro leaf segments and shoots when cultured in Schenk and Hildebrandt medium supplemented with 0.5 mg/l of α -naphthaleneacetic acid. In addition, preliminary phyto-chemical analysis of in vitro root tubers and identification of different secondary metabolites were conducted. Thin layer chromatography and high performance thin layer chromatography analysis of the crude methanolic extracts of the in vitro root tuber identified the presence of lupeol, a bioactive triterpene. Adventitious root tuber induction offers a novel method for the in vitro production of bioactive metabolites that can be scaled up by bioreactors, thus ensuring the conservation and sustainable utilization of H. annulare. The study warrants further scale-up production and pharmacological investigation that can be extended for pharmaceutical needs.

목차

Introduction

Materials and Methods

Results and Discussion

Conclusion

Contribution

Conflict of interest

Announcement for Submission

Acknowledgement

키워드

Adventitious in vitro root tubers Holostemma annulare high performance thin layer chromatography (HPTLC) lupeol Schenk and Hildebrandt (SH) medium