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• 영문명: Combining ex vitro thermotherapy with shoot-tip grafting for elimination of virus from potted apple plant
• 발행기관: (사)한국식물생명공학회
• 저자명: 천재안(Jae An Chun) 권지영(Jiyeong Gwon) 이선기(Seon Gi Lee)
• 간행물 정보: 『Journal of Plant Biotechnology』49권 3호, 222~229쪽, 전체 8쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2022.09.30

영문 초록

Apples are the most grown fruit crops in the fruit industry of Korea. However, virus or viroid infection such as apple mosaic virus (ApMV), apple stem grooving capillovirus (ASGV), apple stem pitting virus (ASPV), apple chlorotic leaf spot virus (ACLSV), apple scar skin viroid (ASSVd) causes fruit yield reduction and poor fruit quality. Therefore, in this study, we examined to established an efficient virus-free system to eliminate the most infected ASGV virus in domestic apple orchard. We investigated that the shoot growth rate and the virus removal rate in ASGV infected potted apples that were treated with heat treatment in a growth chamber (constant temperature/humidity device) maintained at 36°C, 38°C and 40°C for 4 weeks. Here we found that the shoot growth rate was the highest in the heat treatment group (36°C) and the virus was removed in the middle and top of the shoot but not in the bottom. The virus was did not removed in the 38°C and 40°C heat treatment group in all section of shoots, and the heat treatment group (40°C) died after 4 weeks of heat treatment without growth of shoots. We performed in vivo shoot-tip grafting using the shoot-tip of potted apple heat-treated at 36 °C, and we also investigated the viability and virus removal rate, which showed 94% viability and 20% virus removal rate. Collectively, our results suggest that it would be possible to produce the virus-free apple plants through heat treatment and shoot-tip grafting

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키워드

Apple stem grooving capillovirus virus-free apple plants heat treatment shoot-tip grafting potted apple