저온 저장 감수성 및 저항성 고구마 품종에서 저온 반응성 단백질체 연구

• 영문명: Proteome analysis of storage roots of two sweet potato cultivars with contrasting low temperature tolerance during storage
• 발행기관: (사)한국식물생명공학회
• 저자명: 김윤희(Yun-Hee Kim) 지창윤(Chang Yoon Ji) 김호수(Ho Soo Kim) 정정성(Jung-Sung Chung) 최성환 곽상수 이증주
• 간행물 정보: 『Journal of Plant Biotechnology』49권 2호, 118~123쪽, 전체 6쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2022.06.30

영문 초록

To obtain information on the molecular mechanism underlying the low temperature tolerance of sweet potato [Ipomoea batatas (L.) Lam], the proteome expressed in the sweet potato cultivar Xushu 15-1 with high cold storage tolerance and in the cultivar Xushu 15-4 with low cold storage tolerance was analyzed using 2-D and MALDI-TOF/TOF analyses. Compared with the control (without cold treatment), four protein spots were newly expressed in Xushu 15-1. The expression level of one protein spot was higher in Xushu 15-4 than in Xushu 15-1. Spot 2, which was newly expressed in Xushu 15-1, was identified as sporamin. Assessment of the change in protein expression levels over 8 weeks in the storage roots of the two cultivars treated at 4°C revealed no significant difference in the expression levels in Xushu 15-1 over time. However, in Xushu 15-4, the expression level of one protein spot increased, while those of four spots decreased. Of the proteins with reduced expression levels, spots 7 and 8 were identified as actin and spots 9 and 10 were identified as fructokinase-like proteins. The present results are expected to enhance the understanding of the complex mechanism underlying the low temperature tolerance of sweet potatoes during storage and can be used to identify candidate genes for the development of new varieties of sweet potatoes with improved low temperature tolerance during cold storage in the future.

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키워드

cold storage proteome sweet potato Xushu 15-1 Xushu 15-4