In vitro propagation of Phaleonopsis hybrid ‘Little gem’ by culturing apical part and axillary bud of flower stalk

• 발행기관: (사)한국식물생명공학회
• 저자명: Mi Young Chung Aung Htay Naing Khadiza Khatun Hyung Geun Ahn Ki Byung Lim Chang Kil Kim
• 간행물 정보: 『Journal of Plant Biotechnology』43권 4호, 438~443쪽, 전체 6쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2016.12.31

영문 초록

The in vitro propagation of the commercially important Phalaeonopsis hybrid `Little gem` was achieved by culturing the apical part and axillary buds excised from flower stalks. The explants were cultured on 5 different basal media: 3.0 g·L-1 Hyponex and 4.0 g·L-1 peptone (H3P4) and Murashige & Skoog (MS) media were shown to be suitable for shoot regeneration. The MS medium supplemented with 5.0 mg·L-1 6-benzylaminopurine (BA) was found to be more efficient for shoot regeneration. However, the number of shoots induced by axillary buds was higher than that induced by the apical part. Incubation of the apical part under darkness for one week, as well as of the explants in the same medium with activated charcoal (AC) 0.5 g·L-1 promoted shoot regeneration and shoot growth; similar growth was not observed with axillary buds.

목차

Introduction

Materials and Methods

Results

Discussion

Acknowledgment

References

키워드

Activated charcoal Dark treatment Micropropagation Plant growth regulator