Overexpression of rice premnaspirodiene oxygenase reduces the infection rate of Xanthomonas oryzae pv. oryzae

• 발행기관: (사)한국식물생명공학회
• 저자명: Marjohn C. Niñ o Jae-Young Song Franz Marielle Nogoy Me-Sun Kim Yu Jin Jung Kwon-Kyoo Kang Illsu
• 간행물 정보: 『Journal of Plant Biotechnology』43권 4호, 422~431쪽, 전체 10쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2016.12.31

영문 초록

Plants utilize cytochrome P450, a large superfamily of heme-containing mono-oxygenases, in the synthesis of lignins, UV protectants, pigments, defense compounds, fatty acids, hormones, and signaling molecules. Despite the overwhelming assortment of rice P450 accession numbers in the database, their functional studies are lacking. So far, there is no evidence involving rice P450 in disease immunity. Most of our understanding has been based on other plant systems that are mostly dicot. In this study, we isolated the cytochrome P450 (OsCYP71) in rice, and screened the gene using gain-of-function technique. The full-length cDNA of OsCYP71 was constitutively overexpressed using the 35S promoter. We then explored the functions of OsCYP71 in the rice - Xanthomonas oryzae pv. oryzae pathosystem. Using the gene expression assays, we demonstrate the interesting correlation of PR gene activation and the magnitude of resistance in P450-mediated immunity.

목차

Introduction

Materials and Methods

Results

Discussion

Acknowledgments

References

키워드

Cytochrome P450 Premnaspirodiene oxygenase Rice Xanthomonas oryzae pv. oryzae