배 ‘원황’의 재분화와 발근에 미치는 식물생장조절물질과 탄소원의 영향

• 영문명: Effect of plant growth regulators and carbon source on the shoot regeneration and rooting of ‘Wonhwang’ pear (Pyrus pyrifolia L.)
• 발행기관: (사)한국식물생명공학회
• 저자명: 김세희(Se Hee Kim) 박서준(Seo Jun Park) 조강희(Kang Hee Cho) 이한찬(Han Chan Lee)
• 간행물 정보: 『Journal of Plant Biotechnology』43권 4호, 486~491쪽, 전체 6쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2016.12.31

영문 초록

The aim of this research is to establish shoot regeneration system for `Wonhwang` pear (Pyrus pyrifolia L.) using various concentrations of 1-Naphthaleneacetic acid (NAA) 0.01, 0.05, 0.1, 0.5 mg/L in combination with benzylaminopurine (BA) 3, 5, 10 mg/L. Medium containing 4.4 g/L of Murashige and Skoog (MS) medium with vitamins containing 8 g/L of plant agar and 30 g/L of sucrose with NAA 0.05 mg/L and BA 3 mg/L showed 13.3% of shoot regeneration rate. `Wonhwang` showed no root growth on existing rooting media of P. pyrifolia cv. Niitaka, `Whangkeumbae` and `Bae Yun No. 3`. We evaluated the effect of concentration and kinds of plant growth regulators and carbon source to establish efficient rooting condition for `Wonhwang` pear. In the result of using various concentrations of NAA 0.5 mg/L and 1.0 mg/L in combination with indolebutyric acid (IBA) 3, 5, 10 mg/L, rooting rate of 24% was observed using 1/4 Linsmaier and Skoog (LS) medium supplemented with 7.5 g/L glucose as carbon source and IBA 1.0 mg/L with NAA 1.0 mg/L.

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키워드

Tissue culture Micropropagation Root formation