핵심 Microsatellite 마커를 이용한 한국 콩 품종에 대한 Fingerprinting 분석

• 영문명: DNA fingerprinting analysis for soybean (Glycine max) varieties in Korea using a core set of microsatellite marker
• 발행기관: (사)한국식물생명공학회
• 저자명: 권용삼(Yong-sham Kwon)
• 간행물 정보: 『Journal of Plant Biotechnology』43권 4호, 457~465쪽, 전체 9쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2016.12.31

영문 초록

Microsatellites are one of the most suitable markers for identification of variety, as they have the capability to discriminate between narrow genetic variations. The polymorphism level between 120 microsatellite primer pairs and 148 soybean varieties was investigated through the fluorescence based automatic detection system. A set of 16 primer pairs showed highly reproducible polymorphism in these varieties. A total of 204 alleles were detected using the 16 microsatellite markers. The number of alleles per locus ranged from 6 to 28, with an average of 12.75 alleles per locus. The average polymorphism information content (PIC) was 0.86, ranging from 0.75 to 0.95. The unweighted pair group method using the arithmetic averages (UPGMA) cluster analysis for 148 varieties were divided into five distinctive groups, reflecting the varietal types and pedigree information. All the varieties were perfectly discriminated by marker genotypes. These markers may be useful to complement a morphological assessment of candidate varieties in the DUS (distinctness, uniformity and stability) test, intervening of seed disputes relating to variety authentication, and testing of genetic purity in soybean varieties.

목차

서 론

재료 및 방법

결과 및 고찰

적 요

사 사

References

키워드

Cluster analysis Genetic diversity Molecular marker Simple sequence repeat Variety identification