In-vitro Cytotoxicity Testing of Polyethylene and Polystyrene Cup Noodle Containers

• 영문명: In-vitro Cytotoxicity Testing of Polyethylene and Polystyrene Cup Noodle Containers
• 발행기관: 조선대학교 의학연구원
• 저자명: Eun Jeong Kim Young Jin Jeon
• 간행물 정보: 『The Medical Journal of Chosun University』제39권 제4호, 250~254쪽, 전체 5쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2014.12.31

영문 초록

Polyethylene and polystyrene are frequently used resins for making food container including cup noodle bowls. In the present study, we investigated the potential risks of polyethylene and polystyrene cup noodle containers by cytotoxicity tests such as agar diffusion assay, filter diffusion assay, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. We exposed CCL-1 mouse fibroblast cell line to the polyethylene and polystyrene cup noodle containers indirectly through agar gel. We also used eluents derived from the cup noodle containers by storing them for 24 h at 70℃ in liquids, such as distilled water or dimethylsulfoxide (DMSO). Both polyethylene and polystyrene cup noodle container specimen were found to be non-cytotoxic in agar diffusion assay as well as filter diffusion assay. The eluents from polystyrene using DMSO showed slight cytotoxicity in MTT assay.

목차

Introduction

Materials and Methods

Results

Discussion

키워드

Polyethylene Polystyrene Agar diffusion method