국소마취제가 Synaptosomal Plasma Membrane Vesicles의 유동성에 미치는 영향

• 영문명: Effects of Local Anesthetics on the Fluidity of Synaptosomal Plasma Membrane Vesicles Isolated from Bovine Brain
• 발행기관: 대한약리학회
• 저자명: 윤일(Il Yun) 한석규(Suk Kyu Han) 백승완(Seung Wan Baik) 김남홍(Nam Hong Kim) 강정숙(Jung Sook Kang) 정준기(Joong Ki C
• 간행물 정보: 『대한약리학잡지』제24권 제1호, 43~52쪽, 전체 10쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1988.06.30

국문 초록

국소마취제의 약리학적 작용기전을 탐구키 위하여 소의 신선한 대뇌피질로부터 synaptosomal plasma membrane vesicles(SPMV)를 분리한 후 그 소수성 중심부의 microviscosity에 미치는 국소마취제의 영향을 pyrene 형광 probe법으로 측정한 결과 lidocaine HCI과 procaine HCI이 microviscosity를 낮춘다는 것을 알았고 그 정도는 procaine HCI에 비하여 tidocaine.HCI이 더욱 컸다. 또 국소마취 제 가 dimyristoylphosphatidylcholine (DMPC) multilamellar liposomes의 상전 이온도를 낮추며 cooperative unit크기를 감소시킨다는 것도 시차 열량분석법으로 알게 되었다. 상전이온도와 cooperative unit 크기의 감소 정도는 dibucaine HCI>tetracaine HCI>lidocaine HCI > procaine HCI의 순이었다.

영문 초록

To elucidate the mechanism of action of local anesthetics, the effects of local anethetics on the microenvironment of the lipid bilayers of synaptosomal plasma membrane vesicles (SPMV) isolated from bovine brain and dimyristoylphosphatidylcholine (DMPC) multilamellar liposomes were investigated employing the intermolecular excimer fluorescence technique and differential scanning calorimetry (DSC). The relative intensities of excimer and monomer fluorescence of pyrene are a simple linear function of the viscosity of a homologous series of solvents. The microviscosity(η)of the hydrocarbon region of SPMV was measured by this method and the value was 57.3 ± 5.3 cP at 37˚C. In the presence of lidocaine-HCl and procaine-HCl, the values decreased to 46.5 ± 5.1 cP and 54.7 ± 4.8 cP, respectvely. The differential scanning thermograms of DMPC multilamellar liposomes showed that local anesthetics significantly lowered the phase transition temperature, broadened the thermogram peaks, and reduced the size of the cooperative unit. These results indicate that local anesthetics have significant fluidizing effects on biomembranes and perturbation of membrane lipids may produce some, but not all, of their pharmacological actions.

키워드

Local anesthetics Membran fluidity Pyrene excimer fluorescene Differential scanning calorimetry