k - Casein , GMP , Sialic Acid 가 한우송아지 Rotavirus 와 제주도 Bovine Rotavirus ( JBR ) 의 MA - 104 세포감염에 미치는 영향

• 발행기관: 한국축산식품학회
• 저자명: 유제현 김종헌 유대환 신원선 지병주 송진욱 김세민 박범석
• 간행물 정보: 『Food Science of Animal Resources』Korean Journal for Food Science of Animal Resources 제22권 제3호, 274~280쪽, 전체 7쪽
• 주제분류: 농수해양 > 식품과학
• 파일형태: PDF
• 발행일자: 2002.09.30

영문 초록

This study was conducted to investigate inhibitory effects of κ-casein, GMP and sialic acid addition on the infection of MA-104 cells by S97(Korean native cattle rotavirus) and JBR(Jeju island bovine rotavirus). MA-104 cells on incomplete M199 were infected with domestically separated S97 and JBR, activated by incubating at 37℃ for 6 days, and analyzed for the titer of rotavirus. κ-casein, GMP and sialic acid added MA-104 culture infected by activated S97 and JBR were incubated for 15 hours and stained by the AEC stainning method. The number of infected cells were counted on microscope. The titer of S97 and JBR was 2.5×10exp(7) and 2.0×10exp(6) PFU/ml, respectively. The inhibition level against cell infection by S97 was 97.4% for 2000μM of κ-casein and 97.44% for 2000μM of GMP. The inhibition level against cell infection by JBR was 99.52% for 2000μM of κ-casein and 99.78% for 2000μM of GMP. The inhibition level against cell infection by S97 and JBR was 3.85 and 3.63% for 2000μM of sialic acid, respectively. The high inhibitory effects (over 97%) of κ-casein and GMP against infection of MA-104 cells with S97 and JBR indicated great potentials for the use of κ-casein and GMP in the treatment of calf or infant caused by rotavirus.