Roles of ERK and NF-ՊB in Interleukin-8 Expression in Response to Heat Shock Protein 22 in Vascular Smooth Muscle Cells

• 영문명: Roles of ERK and NF-ՊB in Interleukin-8 Expression in Response to Heat Shock Protein 22 in Vascular Smooth Muscle Cells
• 발행기관: 대한생리학회-대한약리학회
• 저자명: Seung-Hun Kang Ji-Hyuk Lee Kyung-Ha Choi Byung-Yong Rhim Koanhoi Kim
• 간행물 정보: 『The Korean Journal of Physiology & Pharmacology』제12권 제4호, 171~176쪽, 전체 6쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 2008.01.01

영문 초록

Heat shock proteins (HSPs) serve as molecular chaperones and play a role in cell protection from damage in response to stress stimuli. The aim of this article is to investigate whether HSP22 affects IL-8 expression in vascular smooth muscle cells (VSMCs), and which cellular factors are involved in the HSP-mediated IL-8 induction in that cell type in terms of mitogen activated protein kinase (MAPK) and transcription element. Exposure of aortic smooth muscle cells (AoSMCs) to HSP22 not only enhanced IL-8 release but also induced IL-8 transcript via promoter activation. HSP22 activated ERK and p38 MAPK in AoSMCs. HSP22-induced IL-8 release was inhibited by U0126, but not by SB202190. A mutation in the IL-8 promoter region at the binding site of NF-ՊB, but not AP-1 or C/EBP, impaired promoter activation in response to HSP22. Delivery of IՊB, but not dominant negative c-Jun, lowered HSP22-induced IL-8 release from AoSMCs. These results suggest that HSP22 induces IL-8 in VSMCs via ERK1/2, and that transcription factor NF-kB may be required for the HSP22-induced IL-8 up-regulation.

키워드

Heat shock protein Interleukin-8 Vascular smooth muscle cell