The Inhibition of TREK2 Channel by an Oxidizing Agent, 5,5 -dithio- bis (2-nitrobenzoic acid), via Interaction with the C-terminus Distal to the 353rd Amino Acid

• 영문명: The Inhibition of TREK2 Channel by an Oxidizing Agent, 5,5 -dithio- bis (2-nitrobenzoic acid), via Interaction with the C-terminus Distal to the 353rd Amino Acid
• 발행기관: 대한생리학회-대한약리학회
• 저자명: Kyoung Sun Park Hyoweon Bang Eun-Young Shin Chan Hyung Kim Yangmi Kim
• 간행물 정보: 『The Korean Journal of Physiology & Pharmacology』제12권 제4호, 211~215쪽, 전체 5쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 2008.01.01

영문 초록

TREK (TWIK-RElated K^＋ channels) and TRAAK (TWIK-Related Arachidonic acid Activated K^＋ channels) were expressed in COS-7 cells, and the channel activities were recorded from inside-out membrane patches using holding potential of −40 mV in symmetrical 150 mM K^＋ solution. Intra</SUP>cellular application of an oxidizing agent, 5,5 -dithio-bis (2-nitrobenzoic acid) (DTNB), markedly decreased the activity of the TREK2, and the activity was partially reversed by the reducing agent, dithiothreitol (DTT). In order to examine the possibility that the target sites for the oxidizing agents might be located in the C-terminus of TREK2, two chimeras were constructed: TREK2 (1-383)/TASK3C and TREK2 (1-353)/TASK3C. The channel activity in the TREK2 (1-383)/TASK3C chimera was still inhibited by DTNB, but not in the TREK2 (1-353)/TASK3C chimera. These results indicate that TREK2 is inhibited by oxidation, and that the target site for oxidation is located between the amino acid residues 353 and 383 in the C-terminus of the TREK2 protein.

키워드

TREK2 Oxidizing agent 5,5 -dithio-bis(2-nitrobenzoic acid) (DTNB) dithiothreitol (DTT) C-terminus Two-pore domain K^＋ channel K_2P