Characterization of Ionic Currents in Human Neural Stem Cells

• 영문명: Characterization of Ionic Currents in Human Neural Stem Cells
• 발행기관: 대한생리학회-대한약리학회
• 저자명: Chae Gil Lim Sung-Soo Kim Haeyoung Suh-Kim Young-Don Lee Seung Cheol Ahn
• 간행물 정보: 『The Korean Journal of Physiology & Pharmacology』제12권 제4호, 131~136쪽, 전체 6쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 2008.01.01

영문 초록

The profile of membrane currents was investigated in differentiated neuronal cells derived from human neural stem cells (hNSCs) that were obtained from aborted fetal cortex. Whole-cell voltage clamp recording revealed at least 4 different currents: a tetrodotoxin (TTX)-sensitive Na⁺ current, a hyperpolarization-activated inward current, and A-type and delayed rectifier-type K⁺ outward currents. Both types of K⁺ outward currents were blocked by either 5 mM tetraethylammonium (TEA) or 5 mM 4-aminopyridine (4-AP). The hyperpolarization-activated current resembled the classical K⁺ inward current in that it exhibited a voltage-dependent block in the presence of external Ba²⁺ (30ՌM) or Cs⁺ (3ՌM). However, the reversal potentials did not match well with the predicted K⁺ equilibrium potentials, suggesting that it was not a classical K⁺ inward rectifier current. The other Na⁺ inward current resembled the classical Na⁺ current observed in pharmacological studies. The expression of these channels may contribute to generation and repolarization of action potential and might be regarded as functional markers for hNSCs-derived neurons.

키워드

Human neural stem cells TTX-sensitive Na⁺ current A-type delayed rectifier hyperpolarization-activated inward current